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dntps without dctp  (New England Biolabs)


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    Structured Review

    New England Biolabs dntps without dctp
    Dntps Without Dctp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2847 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dctp/NEBuffer+2/bio_rxiv__64898__2026__04__16__718949-239-19-34
    Average 99 stars, based on 2847 article reviews
    dntps without dctp - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales.
    Article Snippet: The DNA was dissolved in 40 μL of 1 × NEBuffer 2 containing 0.1 mg/mL RNase A and incubated at 37°C for 30 min. .. Subsequently, 30 μL of DNA obtained from the above purification was mixed with 4.5 μL of 10 × NEBuffer 2, 0.375 μL of 20 mg/mL BSA (Thermo Fisher Scientific), 3.75 μL of 2 mM dCTP, 0.375 μL of 3U/μL T4 DNA Polymerase (NEB), and 36 μL of Milli-Q water to a total volume of 75 μL and then incubated at 20°C for 4 h to remove biotin from unligated free DNA ends. .. The second DNA purification was immediately followed by using a DNA Cycle-Pure Kit (Omega Biotek, Norcross, USA).

    Article Title: Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse Brain
    Article Snippet: .. For each 25 μL purified sublibrary from the previous step, 3 μL 10x TdT Buffer and 1 μL 1 mM dCTP (NEB, N0446S) were added and the reaction was incubated at 95°C for 5 min, and then immediately chilled on ice for 5 min. Next, 1 μL Terminal Transferase (NEB, M0315L) was added, and the reaction mixture was incubated at 37°C for 30 min followed by 75°C for 20 min in a thermocycler. .. Subsequently, 30 μL Anchor Mix [14.4 μL H 2 O, 12 μL 5x KAPA HiFi Fidelity Buffer, 1.2 μL KAPA dNTPs Mix, 1.2 μL 10 μM Anchor-FokI-GH oligo, and 1.2 μL KAPA HiFi HotStart DNA Polymerase (Roche, KK2502)] was added to each reaction and the following thermocycling steps were performed in a thermocycler: Step 1, 98°C x 3 min; Step 2, 98°C x 15 s, 47°C x 1 min, 68°C x 2 min, 47°C x 1 min, 68°C x 2 min, repeat for a total of 16 cycles; Step 3, 72°C x 10 min; Step 4, hold at 12°C.

    Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales
    Article Snippet: The DNA was dissolved in 40 μL of 1 × NEBuffer 2 containing 0.1 mg/mL RNase A and incubated at 37 °C for 30 min. .. Subsequently, 30 μL of DNA obtained from the above purification was mixed with 4.5 μL of 10 × NEBuffer 2, 0.375 μL of 20 mg/mL BSA (Thermo Fisher Scientific), 3.75 μL of 2 mM dCTP, 0.375 μL of 3 U/μL T4 DNA Polymerase (NEB), and 36 μL of Milli-Q water to a total volume of 75 μL and then incubated at 20 °C for 4 h to remove biotin from unligated free DNA ends. .. The second DNA purification was immediately followed by using a DNA Cycle-Pure Kit (Omega Bio-tek, Norcross, USA).

    Incubation:

    Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales.
    Article Snippet: The DNA was dissolved in 40 μL of 1 × NEBuffer 2 containing 0.1 mg/mL RNase A and incubated at 37°C for 30 min. .. Subsequently, 30 μL of DNA obtained from the above purification was mixed with 4.5 μL of 10 × NEBuffer 2, 0.375 μL of 20 mg/mL BSA (Thermo Fisher Scientific), 3.75 μL of 2 mM dCTP, 0.375 μL of 3U/μL T4 DNA Polymerase (NEB), and 36 μL of Milli-Q water to a total volume of 75 μL and then incubated at 20°C for 4 h to remove biotin from unligated free DNA ends. .. The second DNA purification was immediately followed by using a DNA Cycle-Pure Kit (Omega Biotek, Norcross, USA).

    Article Title: Construction of a multilayer fluorescence-encoded single quantum dot-based FRET nanosensor for simple and sensitive monitoring of circular RNA in clinical tissues.
    Article Snippet: Circular RNAs (circRNAs) represent a new category of evolutionarily conserved covalent-closed RNAs with critical roles in tumorigenesis and progression of various cancers.. The accurate and sensitive monitoring of circRNAs is essential for early disease diagnosis and therapeutic monitoring.. Herein, we construct a multilayer fluorescence encoding-mediated single QD-based Förster resonance energy-transfer (FRET) nanosensor for the simple and sensitive monitoring of circRNA in breast tumor cells and clinical tissues.

    Article Title: Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse Brain
    Article Snippet: .. For each 25 μL purified sublibrary from the previous step, 3 μL 10x TdT Buffer and 1 μL 1 mM dCTP (NEB, N0446S) were added and the reaction was incubated at 95°C for 5 min, and then immediately chilled on ice for 5 min. Next, 1 μL Terminal Transferase (NEB, M0315L) was added, and the reaction mixture was incubated at 37°C for 30 min followed by 75°C for 20 min in a thermocycler. .. Subsequently, 30 μL Anchor Mix [14.4 μL H 2 O, 12 μL 5x KAPA HiFi Fidelity Buffer, 1.2 μL KAPA dNTPs Mix, 1.2 μL 10 μM Anchor-FokI-GH oligo, and 1.2 μL KAPA HiFi HotStart DNA Polymerase (Roche, KK2502)] was added to each reaction and the following thermocycling steps were performed in a thermocycler: Step 1, 98°C x 3 min; Step 2, 98°C x 15 s, 47°C x 1 min, 68°C x 2 min, 47°C x 1 min, 68°C x 2 min, repeat for a total of 16 cycles; Step 3, 72°C x 10 min; Step 4, hold at 12°C.

    Article Title: An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales
    Article Snippet: The DNA was dissolved in 40 μL of 1 × NEBuffer 2 containing 0.1 mg/mL RNase A and incubated at 37 °C for 30 min. .. Subsequently, 30 μL of DNA obtained from the above purification was mixed with 4.5 μL of 10 × NEBuffer 2, 0.375 μL of 20 mg/mL BSA (Thermo Fisher Scientific), 3.75 μL of 2 mM dCTP, 0.375 μL of 3 U/μL T4 DNA Polymerase (NEB), and 36 μL of Milli-Q water to a total volume of 75 μL and then incubated at 20 °C for 4 h to remove biotin from unligated free DNA ends. .. The second DNA purification was immediately followed by using a DNA Cycle-Pure Kit (Omega Bio-tek, Norcross, USA).



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    Image Search Results


    Xenopus egg extract recapitulates 5-aza-dCTP-induced SUMOylated DNMT1 accumulation on chromatin. (A) Xenopus sperm nuclei were incubated in egg extracts supplemented with or without 50 μM 5-aza-dCTP for indicated periods. Isolated chromatins were analyzed by immunoblotting with the indicated antibodies. (B) Immunoblotting results using indicated antibodies following DNMT1 immunoprecipitation of chromatin treated or untreated with 5-aza-dCTP for 60 min. The input results were shown in the lower panels.

    Journal: Journal of Biochemistry

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1

    doi: 10.1093/jb/mvag011

    Figure Lengend Snippet: Xenopus egg extract recapitulates 5-aza-dCTP-induced SUMOylated DNMT1 accumulation on chromatin. (A) Xenopus sperm nuclei were incubated in egg extracts supplemented with or without 50 μM 5-aza-dCTP for indicated periods. Isolated chromatins were analyzed by immunoblotting with the indicated antibodies. (B) Immunoblotting results using indicated antibodies following DNMT1 immunoprecipitation of chromatin treated or untreated with 5-aza-dCTP for 60 min. The input results were shown in the lower panels.

    Article Snippet: For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) or with 100 μM GSK-3484862 (MedChemExpress).

    Techniques: Incubation, Isolation, Western Blot, Immunoprecipitation

    DAXX accumulates on DNMT1-trapped chromatin in a SUMO-dependent manner identified by CHROMASS. (A) Volcano plot showing chromatin mass spectrometry analysis of protein recruitment to chromatins treated with or without 5-aza-dCTP ( n = 3 technical replicates). Proteins highlighted in blue were identified in the comparison between 5-aza-dCTP+UBC9-DN and control groups and represent factors whose accumulation on chromatin is associated with SUMOylation. (B) Scatter plot showing protein abundance ratios derived from indicated comparisons in the axis titles.

    Journal: Journal of Biochemistry

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1

    doi: 10.1093/jb/mvag011

    Figure Lengend Snippet: DAXX accumulates on DNMT1-trapped chromatin in a SUMO-dependent manner identified by CHROMASS. (A) Volcano plot showing chromatin mass spectrometry analysis of protein recruitment to chromatins treated with or without 5-aza-dCTP ( n = 3 technical replicates). Proteins highlighted in blue were identified in the comparison between 5-aza-dCTP+UBC9-DN and control groups and represent factors whose accumulation on chromatin is associated with SUMOylation. (B) Scatter plot showing protein abundance ratios derived from indicated comparisons in the axis titles.

    Article Snippet: For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) or with 100 μM GSK-3484862 (MedChemExpress).

    Techniques: Mass Spectrometry, Comparison, Control, Quantitative Proteomics, Derivative Assay

    DAXX interacts with SUMOylated DNMT1 on chromatin through SIMs. (A) The multiple sequence alignment of DAXX from different indicated species. DAXX contains SUMO-interacting motifs (SIMs), four-helix bundle (4HB), histone binding domain (HBD) and acidic residue-rich domain ( ad ). (B) Xenopus sperm nuclei were incubated in egg extracts supplemented with buffer, 5-aza-dCTP or 5-aza-dCTP and SUMO inhibitor ML-792. Isolated chromatin was analyzed by immunoblotting with indicated antibodies. (C) Xenopus sperm nuclei were incubated in mock-depleted or DNMT1-depleted extracts in the presence of buffer or 5-aza-dCTP. Isolated chromatin was analyzed by immunoblotting with indicated antibodies. (D) 3× FLAG-tagged xDAXX WT, 3× FLAG-tagged xDAXX SIM1 mutants (I7AI8A point mutant or 1–14 deletion mutant) or 3× FLAG-tagged xDAXX SIM2 mutants (V911A/I912A point mutant or 909–916 deletion mutant) were added to DAXX-depleted extracts. (E) Xenopus sperm nuclei were incubated in mock-depleted or DAXX-depleted extracts supplemented with xDAXX WT, xDAXX SIM1 mutants or xDAXX SIM2 mutants in the presence of buffer or 5-aza-dCTP.

    Journal: Journal of Biochemistry

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1

    doi: 10.1093/jb/mvag011

    Figure Lengend Snippet: DAXX interacts with SUMOylated DNMT1 on chromatin through SIMs. (A) The multiple sequence alignment of DAXX from different indicated species. DAXX contains SUMO-interacting motifs (SIMs), four-helix bundle (4HB), histone binding domain (HBD) and acidic residue-rich domain ( ad ). (B) Xenopus sperm nuclei were incubated in egg extracts supplemented with buffer, 5-aza-dCTP or 5-aza-dCTP and SUMO inhibitor ML-792. Isolated chromatin was analyzed by immunoblotting with indicated antibodies. (C) Xenopus sperm nuclei were incubated in mock-depleted or DNMT1-depleted extracts in the presence of buffer or 5-aza-dCTP. Isolated chromatin was analyzed by immunoblotting with indicated antibodies. (D) 3× FLAG-tagged xDAXX WT, 3× FLAG-tagged xDAXX SIM1 mutants (I7AI8A point mutant or 1–14 deletion mutant) or 3× FLAG-tagged xDAXX SIM2 mutants (V911A/I912A point mutant or 909–916 deletion mutant) were added to DAXX-depleted extracts. (E) Xenopus sperm nuclei were incubated in mock-depleted or DAXX-depleted extracts supplemented with xDAXX WT, xDAXX SIM1 mutants or xDAXX SIM2 mutants in the presence of buffer or 5-aza-dCTP.

    Article Snippet: For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) or with 100 μM GSK-3484862 (MedChemExpress).

    Techniques: Sequencing, Binding Assay, Residue, Incubation, Isolation, Western Blot, Mutagenesis

    DAXX promotes SUMOylation of chromatin-trapped DNMT1. (A) Immunoblotting results of egg extracts using indicated antibodies. 3× FLAG-tagged xDAXX WT was added to xDAXX-depleted egg extracts. (B) Chromatin fractions were isolated from mock-depleted or xDAXX-depleted egg extracts treated or untreated with 5-aza-dCTP for the indicated times, with or without adding 3× FLAG-tagged xDAXX. DNMT1 SUMOylation was assessed by immunoblotting using anti-DNMT1 antibodies.

    Journal: Journal of Biochemistry

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1

    doi: 10.1093/jb/mvag011

    Figure Lengend Snippet: DAXX promotes SUMOylation of chromatin-trapped DNMT1. (A) Immunoblotting results of egg extracts using indicated antibodies. 3× FLAG-tagged xDAXX WT was added to xDAXX-depleted egg extracts. (B) Chromatin fractions were isolated from mock-depleted or xDAXX-depleted egg extracts treated or untreated with 5-aza-dCTP for the indicated times, with or without adding 3× FLAG-tagged xDAXX. DNMT1 SUMOylation was assessed by immunoblotting using anti-DNMT1 antibodies.

    Article Snippet: For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) or with 100 μM GSK-3484862 (MedChemExpress).

    Techniques: Western Blot, Isolation